Coding
Part:BBa_K3787003:Design
Designed by: Leung Gabriel, Tam Ching Yeung, Wong Chun Hei Group: iGEM21_HK_GTC (2021-09-29)
I.s. MHETase
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 983
Illegal PstI site found at 1012 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 132
Illegal PstI site found at 983
Illegal PstI site found at 1012
Illegal NotI site found at 680 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 983
Illegal PstI site found at 1012 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 983
Illegal PstI site found at 1012
Illegal NgoMIV site found at 607 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We choose E.coli as the bacteria and its strains of DH5[alpha] and C41(DE3). We optimized the sequence for the expression of E.coli.
Source
Ideonella Sakaiensis